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KMID : 0352720100340040321
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Journal of Ginseng Research
2010 Volume.34 No. 4 p.321 ~ p.326
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Production of Hydrolyzed Red Ginseng Residue and Its Application to Lactic Acid Bacteria Cultivation
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Kim Dong-Chung
In Man-Jin
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Abstract
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Enzymatic treatment conditions for red ginseng residue (RGR) were investigated to apply RGR as a microbial medium. Polysaccharide hydrolyase and protease were screened to obtain high solid and carbohydrate yields, and a good degree of carbohydrate hydrolysis. The optimal dosage and reaction time for Viscozyme, the chosen polysaccharide hydrolyase, were found to be 1.0% (w/w) and 3 h, respectively. Of the tested proteases, Flavourzyme, whose optimal dosage was 0.5% (w/w), was selected. Co-treatment with the optimal dosages of Flavourzyme and Viscozyme increased solid yield, carbohydrate yield, and degree of carbohydrate hydrolysis by 76%, 65%, and 1,865%, respectively, over levels in non-treated RGR. The culture characteristics of Leuconostoc mesenteroides strain KACC 91459P grown in enzymatically hydrolyzed red ginseng residue (ERGR) and RGR suspensions were compared. After cultivation for 6 h, the viable cell counts of both cell suspensions rapidly increased to 1.3×10? colony-forming units (CFU)/g. Moreover, while the viable cell population drastically decreased to 2.4×10? CFU/g for cells grown in RGR medium, it was maintained in cells fermented in ERGR medium for 24 h.
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KEYWORD
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Enzymatic hydrolysis, Lactic acid bacteria, Microbial medium, Red ginseng residue
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